大花蕙兰组培快繁技术的研究

Study on the Technical System of Tissue Culture and Rapid Propagation in Cymbidium Grandiflorium

以大花蕙兰的茎尖为外植体,探讨了不同培养基对原球茎增殖、分化和小苗诱导及壮苗生根的影响.结果表明:1)大花蕙兰组织培养的原球茎诱导和增殖都可以用培养基1/2MS+BA1.0mg/L+NAA0.5mg/L;2)适合大花蕙兰组织培养原球茎芽苗诱导的培养基配方为1/2MS+BA1.5mg/L+NAA0.5mg/L;3)适合大花蕙兰壮苗生根的培养基配方为1/2MS+NAA0.1mg/L+GA1.0mg/L的组合.

The effects of inducement of protocorm-like bodies (PLB) in the different medium with stem apex as explants,and of multiplication and differentiation of PLB in different medium with PLB as explants were compared. The results were as follows:1) The best medium for the inducement and multiplication of PLB both were 1/2MS+BA 1.0mg/L+NAA 0.5mg/L,so it could reduce a medium preparation; 2) The best medium for the developing of shoots from PLB was 1/2MS+BA 1.5mg/L+NAA 0.5mg/L;3)The best medium for the developing of roots from PLB was 1/2MS+NAA 0.1mg/L+GA1.0mg/L.