摘要
利用筛选出的8对引物对56份来源于不同国家和地区的栽培萝卜种质的亲缘关系进行了AFLP分析,共扩增出327条带,其中多态性带128条,多态性位点百分率39.1%,显示出栽培萝卜种质之间存在着较丰富的遗传多样性。系统聚类分析将供试材料分为5类9组,主坐标分析将其分为4类7组,2种分类方法所获结果基本一致。基于分子标记的分类与种质的表型基本吻合。
Phylogenesis of 56 cultivated radish germplasm from different countries or regions was analyzed with amplified fragment length polymorphism (AFLP). A total of 327 bands were detected with 8 pairs of informative AFLP primers among the accessions tested. Of them, 128 bands (39.1%) were polymorphic, indicating abundant genetic diversity existing among the cultivated radish. The 56 cultivars were clustered into 5 groups and 9 subgroups by cluster analysis, and 4 groups and 7 subgroups by principal coordinate analysis. The two methods above exhibited similar phylogenesis among the tested cultivars. The results from molecular classification were almost coincided with germplasm phenotypes.
出处
《中国农业科学》
CAS
CSCD
北大核心
2005年第5期1017-1023,共7页
Scientia Agricultura Sinica
基金
农业部农作物资源保护资助项目(NB03-6)
"十五"国家科技攻关项目资助(2004BA525B08)
关键词
萝卜
种质资源
亲缘关系
AFLP
聚类分析
主坐标分析
Radish
Raphanus sativus L.
AFLP
Cluster analysis
Principal coordinate analysis
Phylogenesis
