流式细胞术DNA倍体分析在恶性胸腔积液中诊断的价值

The value of DNA ploidy analysis by flow cytometry in diagnosis of malignant pieural effusion.

目的 探讨流式细胞术(FCM)DNA倍体分析在恶性胸腔积液中的诊断价值。方法 以102例胸腔积液患者(其中恶性45例,良性57例)的胸水分别作流式细胞术DNA倍体分析,癌胚抗原(CEA)及脱落细胞学检测。比较三者之间的敏感性、特导性。结果 1.FCM、CEA、脱落细胞学检测在良、恶性胸腔积液中的阳性率分别为3.5％、82.2％、15.8％、71.1％、7.0％、46.7％,三种方法均有显著性差异(P值均<0.05)。2.FCM、CEA、脱落细胞学检测的敏感性分别为82.2％、71.1％、46.7％,FCM与CEA之间无显著性差异(P>0.05),FCM与脱落细胞学之间差异有显著性(P<0.05)。3.FCM、CEA、脱落细胞学检测的特异性分别为96.5％、84.2％、94.7％,FCM与CEA之间有显著性差异(P<0.05),FCM与脱落细胞学之间无显著性差异(P<0.05)。结论 流式细胞术DNA倍体分析在恶性胸腔积液中其敏感性优于脱落细胞学检测,特异性优于CEA检测,是鉴别良、恶性胸腔积液较理想的检测方法。

Objeative To explore the value of DNA ploidy analysis by flow eytometry in diagnosis of malignant pleural effusiom. Methods Flow cytometry (FCM), carcinoembryonic antigen(CEA) quantitation, and exfoliative cytologic examination were used to detect 102 patients with pleural effusion(malignant 45, benign 57 respeetively), and compare their sensitivity and specificity. Results l.The positive rate is 3.5%, 82.2%, 15.8%. 71. 1% , 7.0%, 46.7% with FCM, CEA and exfoliative cytologic examination respectively. There was significant difference between benign and malignant pleural effusion (P < 0.05) . 2. FCM, CEA and exfoliative cytologic examination' s sensitivity is 82. 2% , 71.1%, 46. 7% . There is no significant difference between FCM and CEA (P > 0.05) . There was significant difference between FCM and exfoliative cytologic examinatiln(P < 0.05) . 3. FCM, CEA and exfoliative cytologic examination' s specificity is 96. 5% , 84. 2% , 94.7% . There was significant difference between FCM and CEA(P < 0.05) . no significant difference between FCM and exfoliative cytologic examination (P > 0.05) . Conclusion DNA ploidy analysis by flow cytometry has higher sensitivity and specificity in disinguishing malignant and benign pleural effusion and is a better method.