杂色曲霉素对体外小鼠腹腔巨噬细胞白细胞介素12表达与分泌的影响

Effect of sterigmatocystin on interleukin-12 expression and secretion of murine peritoneal macrophage cells in vitro

目的 探讨杂色曲霉素(ST)对巨噬细胞免疫功能的影响。方法 分别采用半定量逆转录聚合酶链反应及酶联免疫吸附法,研究0 .12 5 ,0 .2 5 ,0 .5 ,1.0和2 .0mg·L- 1ST预处理对小鼠腹腔巨噬细胞白细胞介素(IL) 12mRNA表达及其蛋白分泌的影响。结果 在0 .12 5～1.0mg·L- 1范围内,ST处理对小鼠腹腔巨噬细胞IL 12p4 0和IL 12p35mRNA的表达均有一定的抑制作用。但在蛋白水平,对IL 12分泌的影响不明显。当ST浓度达到2 .0mg·L- 1时,可明显抑制IL 12p4 0mRNA的表达及IL 12的分泌。结论 ST可抑制巨噬细胞IL 12的表达或分泌,提示ST可能在一定程度上对机体的细胞免疫功能产生不利影响。

AIM To explore the effect of sterigmatocystin (ST) on immunologic function of macrophages. METHODS The secretion and expression of interleukin(IL)-12 of murine peritoneal macrophages after ST pretreatment at different concentrations(0.125,0.25,0.5,1.0 and 2.0 mg ·L -1 ) were studied with ELISA and semi-quantitative RT-PCR method, respectively. RESULTS In the concentration range 0.125-1.0 mg·L -1 , ST could inhibit the expression of IL-12 p40 and IL-12 p35 at mRNA level, but no effect on the secretion of IL-12 protein was found. ST at 2.0 mg·L -1 could significantly inhibit the expression of IL-12 p40 mRNA and the secretion of IL-12 protein. CONCLUSION ST could inhibit the expression or secretion of IL-12 of murine peritoneal macrophages.