树突细胞联合细胞因子诱导的杀伤细胞对耐药K562细胞的体外杀伤作用

Effect of dendritic cells co-cultured with cytokine induced killer cells on cytotoxicity against drug resistant K562 cells

目的研究细胞因子诱导的杀伤(CIK)细胞与同源树突细胞(DC)共培养后CIK细胞的增殖活性及表型的变化,并观察其对K562、K562/ADM细胞毒作用的影响。方法采集健康供者外周血单个核细胞(MNC)用于诱导培养CIK细胞及成熟DC,将成熟DC和CIK细胞混合培养,用MTT法检测DCCIK共培养细胞杀伤K562细胞及其耐药株的活性。结果在2.5～20.0效靶比范围内,CIK细胞对K562和K562/ADM细胞的杀伤率分别为(20.0±1.2)%～(61.1±2.2)%和(17.5±2.1)%～(45.2±3.3)%;DCCIK共培养细胞对K562和K562/ADM细胞的杀伤率分别为(25.2±2.3)%～(70.9±4.1)%和(22.4±2.7)%～(62.3±5.0)%。CIK细胞对K562敏感株和耐药株杀伤率的差异无统计学意义,DCCIK细胞对敏感株和耐药株的杀伤作用差异亦无统计学意义;DCCIK细胞对K562和K562/ADM细胞的杀伤活性均高于单纯CIK细胞组,差异有统计学意义(P<0.05)。结论DC与CIK共培养细胞的增殖活性和细胞毒活性高于CIK细胞。

Objective To study the effect of dendritic cells(DC) co-cultured with cytokine induced killer (CIK) cells on cytotoxicity against K562 and K562 drug-resistant (K562/ADM) cells. Methods Peripheral blood mononuclear cells(MNC) isolated from healthy adult donors were induced to obtain CIK cells and DC respectively and then these two kinds of cells were co-cultured. The cytotoxicity of the co-cultured cells against K562 and K562/ADM cells was measured with MTT assay. Results The cytotoxicity CIK cells alone to K562 and K562/ADM cells was(20.0 ±1.2)%-(61.1±2.2)% and (17.5±2.1)%-(45.2± 3.3)% respectively at low effector to target ratios(2.5-20.0). This effect was significantly enhanced by co-culturing with DCs being (25.2±2.3)%-(70.9±4.1)% and (22.4±2.7)%-(62.3±5.0)%. Conclusion CIK cells showed high cytotoxicity against K562 and K562/ADM cells and the activity could be enhanced by co-culturing with DC.