摘要
以小麦悬浮细胞为材料,分别用感染叶锈菌小种165的小麦细胞间隙液(IWF165)和未接菌的小麦细胞间隙液(IWFck)对其进行处理,处理后不同时间点取样进行形态学观察和DNA电泳。结果表明,用IWF165处理后的悬浮细胞可观察到明显的染色质凝聚化、边缘化和细胞核解体等现象;同时DNA电泳结果显示IWF165处理后的细胞基因组DNA发生降解并形成明显的DNA梯状条带。而用IWFck处理的悬浮细胞,其细胞核结构完整,染色质分布均匀;DNA电泳没有出现DNA梯状条带。在感染叶锈菌的小麦细胞间隙液中存在着激发子,它能够诱导小麦悬浮细胞发生程序性死亡。
The suspension cells of wheat were treated with IWF_(165) and IWF_(ck). The cells were observed with fluorescence microscope and made DNA agarose gel electrophoresis at different times after being treated.It was observed in the IWF_(165) treated cells that the nuclear gradually became irregular with highly condensed chromatin and the nuclear envelope underwent disruption and DNA agarose gel electrophoresis showed DNA ladder. But, these phenomena were not observed in the IWF_(ck) treated cells. These results suggest that IWF_(165) contains elicitor and the elicitor can induce PCD of wheat suspenion cells.
出处
《河北农业大学学报》
CAS
CSCD
北大核心
2005年第3期12-16,共5页
Journal of Hebei Agricultural University
基金
河北省教委博士基金资助项目(B2001211)
河北省自然科学基金资助项目(399172
301124
303180)
教育部科学技术研究重点资助项目(03014)
植物生理学与生物化学国家重点实验室开放课题资助(PPB04006)
关键词
小麦悬浮细胞
细胞间隙液
程序性死亡
激发子
suspension cell of wheat
intercellular washing fluid(IWF)
programmed cell death(PCD)
elicitor
