摘要
本实验旨在研究熊果酸对正常人外周血淋巴细胞Th1型细胞因子的影响,并对机制作初步探讨。采用半定量RT-PCR和夹心法ELISA分别检测不同浓度熊果酸对PHA和PMA活化的外周血淋巴细胞的Th1型细胞因子(IFN-γ和IL-2)mRNA的表达及分泌的影响。结果显示Th1型细胞因子mRNA和蛋白的表达水平均随熊果酸浓度的增加而下降(P<0.05),表明熊果酸可能对IFN-γ和IL-2在转录和(或)转录后水平发挥抑制作用,有可能用于自身免疫性疾病和超敏反应性疾病的防治。而熊果酸和地塞米松分子结构相似,可能通过类似机制下调糖皮质激素受体发挥作用。
The effects of ursolic acid on the expression of the Th1 type cytokines in human normal peripheral blood lymphocytes were investigated in the present study, in which the effects of varying concentrations of ursolic acid using DMSO as solvent on the expressions of the Th1 type cytokines(IFN-γ and IL-2) mRNA and secretion of these cytokines in the activated peripheral blood lymphocytes induced by PHA and PMA were determined by semi-quantitative RT-PCR with β-actin as the internal control and sandwich ELISA. It was demonstrated by RT-PCR analysis and ELISA assay that ursolic acid caused a dose-dependent inhibition of IFN-γ and IL-2 secretions with significant significance. The result of this study indicates that the inhibitory effects of ursolic acid on the expression of Th1 type cytokines and their secretion in normal peripheral blood lymphocytes were evident at transcriptional and/or post-transcriptional level. Therefore, ursolic acid may be considered to be used to treat allergic and autoimmune disorders. On account the similarity on structures between ursilic acid and dexamethasone, it may exert its effect as a glucocorticoid through glucocorticoid receptor-mediated manner.
出处
《现代免疫学》
CAS
CSCD
北大核心
2005年第3期235-238,共4页
Current Immunology
