一氧化氮在全肠外营养所致胆汁淤积中的作用

Effect of nitric oxide on total parenteral nutrition induced cholestasis

目的:研究一氧化氮(NO)在全肠外营养(TPN)所致胆汁淤积中的作用。 方法:将24只新生兔分为三组:正常对照组、TPN1周组和TPN2周组。第7或14天后,分别取血及肝组织,以全自动生化仪检测血清肝功能,分别以Griess反应法、分光光度法和原位杂交法测定血清和肝组织中NO水平,肝组织NOS活性和iNOSmRNA表达。 结果:TPN第7天和14天后,血清和肝组织NO水平、肝组织NOS活性和iNOSmRNA表达较正常对照组明显增高(P<0. 05,P<0. 01),TPN2周组较TPN1周组有进一步增高(P<0. 05)。 结论:iNOS产生的NO在TPN引起的淤胆中发挥重要作用。

Objective: To investigate the effects of NO on cholestasis caused by TPN. Methods: 24 newborn rabbits were divided into 3 groups: control group, TPN for 1week group and TPN for 2weeks group. After 7 or 14 days, serum liver function test was determined using automatic biochemical analyzer, NO levels in serum and liver, liver NOS activity and iNOSmRNA expression were determined respectively by the Griess method, spectrophotometric analysis and in situ hybridization. Results: After having received TPN administration for 7 or 14 days, the NO levels of serum and liver, liver NOS activity and iNOSmRNA expression increased significantly than those in control group(P<0.05, P<0.01). Above parameters in TPN 2w group were increased further compared with TPN 1w group(P<0.05). Conclusion: NO produced by iNOS plays an important role in cholestasis induced by TPN.