儿茶酚胺致心肌损伤的实验病理研究

EXPERIMENTAL PATHOLOGICAL STUDY OF MYOCARDIAL DAMAGE INDUCED BY CATECHOLAMINE

大鼠尾静脉滴去甲肾上腺素（20μg／kg／min）后，分别于即刻、7天、30～90天杀死取材，用病理组织学、肌红蛋白免疫组化染色及透射电镜观察心肌损伤。结果：早期可见心肌多灶性坏死、肌红蛋白缺染、肌原纤维断裂溶解，似冠状动脉无阻塞性心肌梗死；中后期可见心肌灶性萎缩及疤痕形成、成纤维细胞增生活跃、新生胶元原纤维伸入坏死心肌内，类似早期心肌病图象。

The experimental myocardial damages were induced in Wistar rats by noradrenaline infusion (20ug/kg body wt/minute)for a period of 90 minutes daily for three consecutive days. The animals weresacrificed few minutes (acute phase), 7 days(subacute phase),and 30~90 days (chronic phase)afterinfusion. Specimens of myocardium were examined by histopathological, immunohistochemical and ultrastructural methods. Multiple focal necrosis, deficient of myoglobin in myocytes, myofibrillar fragmentation and dissolution were observed in the acute phase. The mdrphofogical feature simulated themyocardial infarctinn without coronary occlusion. Focal myocardial atrophy, scar f0rmation of myocardium, proliferation of fibroblasts and infiltraiton of the neo-colleagenous fibrils in necrotic myocytes were observed in the subacute and chronic phase. This feacture simulated the early cardiomyopathy.