牙种植体结合上皮形成的动物实验

Junctional epithelialization of dental implants in animals

目的:观察牙种植体结合上皮形成的组织学过程。方法:HE染色及增殖细胞核抗原(PCNA)免疫组化染色,观察Beagle狗1、3、6、9、12、15、20、30d共8个时间段的牙种植体结合上皮区。结果:种植术后第12d在牙种植体周围结合上皮开始形成,PCNA呈强阳性表达;第15d上皮细胞增生趋于平稳,PCNA表达减弱;第20d有类似天然牙的结合上皮细胞附着,PCNA维持低水平表达。结论:纯钛牙种植体周围能形成类似天然牙的结合上皮。

<Abstrcat> Objective: To detect the histological changes and the expression of proliferating cell nuclear antigen(PCNA) in junctional epithelialization of dental implants. Methods: The junctionalepithelium was detected by HE staining and the PCNA expression was observed byimmunohistochmical staining on the 1st,3 rd,6 th,9 th,12 th,15 th,20 th and 30 th day after implantation in Beagle dogs. Results: The junctionalepithelium began to form and PCNA was expressed positively 12 days after implantation.The epithelial hyperplasia tended to stabilization and the expression ofPCNA weakened drastically 15 days after implantation.The epithelial attachment showed to be similar to that of natural teeth 20 days later. Conclusion:The gingiva epithelium can attach to titanium implants forming junctional epithelium which is similar to the natural teeth.