国人六个高变DNA位点的基因频率分布

ALLELE FREQUENCY DISTRUBUTIONS OF SIX HYPERVARIABLE DNA LOCI IN CHINESE

使用6个单位点探针，用Southern印迹杂交技术研究了国人的D2S44，D17S79，D14S13，D14S1，DXYS14及D18S27等位点的基因频率分布。发现它们的DNA指纹图均具高度多态性，等位基因数27～107个。非父排除率（EPP）分别为0．8407、0．7691、0．9792、0．9040、0．9400及0．8177，累积非父排除率为0．99999；个人识别能力（DP）分别为0.9880、0.9766，0.9996，0.9961，0.9973及0．9338，累积个人识别能力接近1，故可作为亲子鉴定及个人识别非常有力的工具。此外还对单位点探针的优缺点进行了讨论。

Allele frequency distributions of DNA at D2S44, D17S79, D14S1,DXYS14 and D18S27 loci werestudied using single-locus DNA probes with Southern blot technique. It was showed that the DNAprofiling of all of them possess high polymorphism. The number of allele varies from 27 to 107. Theexcluding probabilities of paternity of them were 0. 8407, 0. 7691, 0. 9792, 0. 9040,o, 9400and 0. 8177respectively, the total excluding probability of paternity was 0. 99999; the discriminating power ofthem were 0. 9880,0. 9766,0. 9996, 0. 9961: 0. 9973 and 0. 9338 respectively, the total discriminating power was appoximately to 1. Therefore, the combination of these six hypervariable loci can be anextremely powerful tool for parternity test and personal identiflcation. This can be applied as well todiagnosis of transplanted organ surviving. The advantages and disadventages of single locus DNAprobe are discussed here also.