复合扩增STR位点片段长度多态性的研究

AMPLIFIED FRAGMENT LENGTH POLYMORPHISM ANALYSIS OF THREE STRs LOCI IN CHINESE USING MULIPLEX PCR

本文介绍了以三个短串联重复序列（STR）位点为基础的复合扩增进行个体识别的技术。其特点是在同一扩增体系内对彼此独立的三个STR位点，即HUMTH01[AATG]nHUMFABP[AAT]nHUMARA[AGC]n进行复会扩增，扩增产物用聚丙烯酰胺凝胶电泳分离。经研究发现这三个STR位点具有高度多态性，扩增产物分子量在190～310bP之间，各位点等位基因数分别为6、9、16个，杂合度78．2％、85，0％、89．0％，个体识别率0．918、0．960、0．905，其累积的个体识别率达0．9997，高于pMCT118等位点，且扩增时间短，操作简便，为法医物证检验提供了一种高效的个体识别手段。

The multiplex PCR of three STRs loci {HUMTHO1 [AATG] n, HUMFABP [AAT] n, HUMARA[AGC]n} for personal identification has been descrrbed. The PCR products were analyzed by5M Urea, 8 %PAGE,followd by silver stain. 6, 9, 16 alleles,ranging from 190bp to 310 bp in size, 78.2 %, 85. 0 %, 89. 0 % heterozygosity of three loci were demonstrated. The combined averagee individualizati0n potential was 3/10000 individuals