摘要
本文用Gelatin-Senharose4B亲合层析法提纯人血浆Fn抗原,免疫家兔,收集抗血清,经过去Fn血浆制成的免疫固相吸附剂处理后,制备特异性抗人Fn血清。此抗血清1:200稀释,用于免疫组化PAP、DAB染色法,观察人头皮枪弹创、头皮挫裂创、腹部皮肤切创各4例创缘区Fn含量、分布变化的研究,能反映创线区Fn变化的规律,为法医学创伤的研究,进一步开展定量分析和定量测定,作了抗体准备。
The fibronectin was purified from human plasma by Gelatin-Sepharose 4B affinity chromatography. The rabbits were immunized with the fibronectin. the raw anti-fibronectin sera were absorbed bythe solid phase of the fibronectin free human serum. Then the specific anti-fibronectin sera were obtained. The fibronectins deposited on human bullet wounds, incised wounds as well as on blunt forceinJuries were demonst.ated by PAP immunohistochemical study using diluted anti-fibronectin serum.The results were quite sati factory.
出处
《中国法医学杂志》
CSCD
1994年第4期227-229,共3页
Chinese Journal of Forensic Medicine
基金
国家自然科学基金