明胶分子量分布测定和表征的研究进展(二)

The Research Development of Molecular Weight Dislribution(MWD)and Its Characterization of Gelatin

6.SDS—PAGE(十二烷基硫酸钠-聚丙烯 酰胺凝胶电泳)法 从1959年Raymond和1964年Davis用聚丙烯酰胺凝胶分离人血清蛋白成功后,聚丙烯酰胺凝胶电泳得到了广泛的发展,成为分离、分析蛋白质。

The following points arc reviewed in present paper:1. Introduction.2. The range of gelatin's MWD.3. The important physicochemical characteristics in relation to MWD,viscosity, Bloom value, renaturation capacity, setting point, solubility, etc.4. The MWD determination methods used in past earlier period:viscosimetry, osmometry, supcrccntrifugation, light scattering method, gel permeation chromatography(GPC), etc.5. HPLC:equipment, column, mobile phase, bed, chromatogram, etc.6. SDS-PAGE:tube method, slab method, gel gradient, buffer system, patterns.7. The comparison between HPLC and SDS-PAGE.8. Retrospect and prospect.