口腔癌及癌前病变中脆性组氨酸三联体基因的表达及其意义

Expression and significance of fragile histidine triad in oral cancer and precancerous lesions

目的检测抑癌基因脆性组氨酸三联体基因(FHIT)在口腔鳞状细胞癌(OSCC)及癌前病变中的表达情况,并探讨其在口腔癌发生过程中的作用及其意义。方法采用SP免疫组化方法检测64例OSCC、39例口腔癌前病变、12例正常粘膜中FHIT蛋白情况的表达。结果正常粘膜的FHIT蛋白100%(12/12)高表达;癌前病变组中均为中度和高表达,与正常组相比无统计学差异;64例OSCC中,3例为阴性表达、8例低表达、43例中度和高度表达;OSCC中FHIT蛋白缺乏或减少的比率为17%(11/64),与正常组、癌前病变组相比有显著差异,而与OSCC的分化程度无关。结论FHIT在口腔鳞癌的发生过程中起着一定的作用。

Objective To detect the expression of fragile histidine triad (FHIT) in oral cancer and oral precancerous lesions and investigate the relationship between the FHIT expression and the histopathological changes. Methods Immunohistochemical staining by SP methods was utilized to detect the expression of FHIT in 64 cases of oral squamous cell carcinoma, 39 oral precancerous lesions and 12 normal oral mucosa specimens. Results The positivity rate of FHIT in normal oral membrane was 100% (12/12), and medium and high FHIT expression levels were detected in oral precancerous lesions but without significant difference from that in normal oral mucosa. In 64 oral squamous cell carcinoma specimens, 3 were negative for FHIT expression, 8 had low expression, and the other 43 had moderate and high expression. The rate of negative or low FHIT expression in the oral squamous cell carcinoma was 17% (11/64), which was significantly different from that in the normal oral mucosa and oral precancerous lesions, but the rate was not correlated to the differentiation of the cancer. Conclusion The tumor suppressor gene FHIT plays a role in the pathogenesis of oral squamous cell carcinoma.