摘要
采用重复序列PCR将引物间多种基因序列目标“放大”,继而通过狭缝杂交,用特异基因探针在微量标本中可检测基因的扩增。采用本法对20份食管癌家族标本进行c-myc基因扩增检测,其结果与双PCR法所得一致。
Genomic sequences are amplified,together with a variety of inter-primer gene targets,from a smallamount of samples by repeats PCR. Amplifications of gene(s)were then detected in the amplified productswith specific gene probe (s) by slot blot hybridization. The amplification of c-myc gene was detected among20 specimens from esophageal cancer families with the present method. The result is similar to that obtainedwith double PCR.
出处
《高技术通讯》
CAS
CSCD
1994年第11期1-3,共3页
Chinese High Technology Letters
基金
863计划和世界实验室资助项目
关键词
重复序列
狭缝杂交
基因扩增
食管癌
Repeats PCR,Alu PCR,Slot blot hybridization,Gene amplification