油菜素内酯对卷丹愈伤组织生长及分化的影响

Effect of Brassinolide on Growth and Regeneration of Lilium lancifolium Thunb Callus

以百合科草本植物卷丹为试材,研究了油菜素内酯(BL)对卷丹(LiliumlancifoliumThunb)愈伤组织生长及分化的影响。结果表明:MS+6 BA1 0mg/L+NAA0 1mg/L培养基中添加不同质量浓度的BL(0 001～1 000mg/L),与对照相比,其愈伤组织鲜重增加率、愈伤组织分化率均有显著提高,浓度为0 010mg/L处理愈伤组织鲜重增加率最高,是对照的249%;浓度为0 100mg/L处理愈伤组织分化率最高,是对照的441%。处理组愈伤组织叶绿素含量、蛋白质含量与对照相比均有不同程度的提高,其中最有效的BL质量浓度是0 010mg/L。处理组愈伤组织过氧化物酶活性与对照相比均稍有降低,但差异并不显著。表明BL能促进卷丹愈伤组织的增殖及分化并有保绿作用。

<Abstrcat> Effects of brassinolide on growth and regeneration of Lilium lancifolium thunb callus were studied. The results showed that the ratio of fresh weight and shoot regeneration significantly increased when varied concentrations(0.001～1.000 mg/L) of BL were added into the medium consisting of MS, 6-BA(1.0 mg/L) and NAA (0.1 mg/L). The ratio of fresh weight was the highest (increasing by 249% to ck) when BL concentration reached 0.010 mg/L, but the most reasonable concentration of BL for shoot regeneration was 0.100 mg/L(increased by 441% to ck).Physiological determination showed that chlorophyll content and protein content also increased, and the most effective concentration of BL was 0.010 mg/L. The activity of peroxidase decreased slightly, but the difference was not significant. All these showed that BL can promote the multiplication and regeneration of Lilium lancifolium thunb callus and has the function of keeping green.