甲醛对原代培养大鼠皮质神经元能量代谢的影响

Effect of formaldehyde on energy metabolism in postnatal rat cortex neurons in culture

目的 探讨甲醛对中枢神经系统毒作用的机制。方法 本文对新生大鼠原代培养皮质神经元进行了剂量分别为1,2 ,4 ,8mg L培养基的甲醛暴露实验,以在培养基中添加甲醛的方式染毒,每小时染毒一次,4小时后,用细胞化学方法,以细胞色素氧化酶(cytochromeoxidase ,COX)活力为指标,观察甲醛对神经元能量代谢的影响。结果 与对照组相比,各实验组神经元COX活力均明显降低(P <0. 0 1) ,并呈剂量-效应关系(R值为- 0 .92 ,P <0 . 0 1)。结论 本文结果提示,过量甲醛暴露可降低神经元COX活力,导致能量代谢障碍,进而影响其正常的生理功能。

Objective The mechanism of the effect of formaldehyde on CNS which is much concerned to formaldehyde poisoning was studied. Methods In the present study, incubation of postnatal rat cortex neurons in culture with formaldehyde at 1,2,4,8mg/L (medium) was carried out to evaluate the effect of formaldehyde on energy metabolism. Results The result of cytochemistry showed a significant down-regulation of cytochrome oxidase activity after consecutive formaldehyde treatment for 4 hours compared with the control (P<0.01), the significant dosage-response relationship was also observed(R value is - 0.92, P<0.01). Conclusion The result demonstrates that excessive exposure of formaldehyde can decrease cytochrome oxidase activity in cortex neurons which indicates energy metabolism will be decreased and therefore normal physiology function would be damaged.