摘要
目的 将E.coli中表达的人胞外超氧化物歧化酶(hEC SOD)包涵体进行复性。方法 分离包涵体,用8mol L尿素溶解后进行透析复性。结果 包涵体蛋白可以部分复性,比活达到900U mg protein。结论 透析复性法可以复性EC SOD包涵体。
Objective To renature the inclusion bodies of recombinant human extracellular superoxide dismutase (hEC SOD) expressed in E. coli.Method The inclusion bodies were isolated and renatured with the method of dialysis after dissolved with 8 mol/L urea. Result The inclusion body proteins could be partially renatured, its specific activity was about 900U/mg protein.Conclusions EC SOD inclusion bodies can be renatured by method of dialysis.
出处
《食品与药品》
CAS
2005年第06A期28-30,共3页
Food and Drug
