血红素氧合酶-1在早期腹主动脉瘤中的动态表达

The dynamic expression of heme oxygenase-1 in early abdominal aortic aneurysm in rats

目的探讨早期腹主动脉瘤(AAA)的发病机制。方法建立大鼠腹主动脉瘤模型,于术后3、7、14、28d取材,观察主动脉的扩张情况,弹力纤维染色观察弹力纤维的损伤;原位分子杂交检测动脉壁中血红素氧合酶(HO)1mRNA的动态表达,免疫组织化学染色检测细胞间黏附分子(ICAM)-1及巨噬细胞特异性抗原CD68的蛋白表达。结果在AAA组织中,HO1mRNA表达于3d出现,14d达到高峰,为(33.9±6.9)%,与其他时段相比,差异有统计学意义(P<0.01)。ICAM1及CD68蛋白表达分别于7、14d达到高峰(P<0.01)。结论HO-1在早期腹主动脉瘤组织中表达增强,伴随着弹力纤维损伤和炎性细胞浸润。

Objective To detect the pathogenic mechanism of early abdominal aortic aneurysm (AAA).Methods Experimental AAA model was made by elastase perfusion and the specimens were harvested on the postoperative day 3,7,14 and 28 respectively.The dilatation rate of aorta was calculated.HE staining and Verhoeff-van Gieson staining were performed to observe the elastic fiber disruption and inflammatory cell infiltration,and in situ hybridization was applied to detect the expression of HO-1 mRNA in aortic wall.The protein expression of ICAM-1 and CD68 was detected by immunohistochemical staining.Results After perfusion,the dilation was increased and experimental AAA model successfully established on the postoperative day 7.The HO-1 mRNA expression was detectable on the postoperative day 3,and peaked on the postoperative day 14,which showed significant difference in comparison with other time points (P< 0.01).The expression of ICAM-1 and CD68 protein peaked on the postoperative day 7,14 respectively (P< 0.01).Conclusion HO-1 gene was strongly expressed in early AAA,together with elastic fiber disruption and inflammatory cell infiltration.