厌氧污泥体系脱氢酶活性表征细菌数的研究

Characteristic Bacterial Number of Anaerobic Activated Sludge with Dehydrogenase Activity as Indicator

以硫化钠作还原剂,甲苯为提取溶剂,用氯化三苯基四氮唑(TTC)法测定污泥脱氢酶活性,探讨了脱氢酶活性与细菌数对数(lgABN)的相关性,并建立了脱氢酶活性表征细菌数的数学模型。结果表明:在lgABN为7.5～12范围内,脱氢酶表征的污泥活性与细菌数对数(lgABN)呈明显线性相关,从理论上分析了其惟一线性相关性;在细菌数的对数达到7.5～12的对数期、稳定期与衰亡期,脱氢酶活性换算成对应细菌数所绘制的曲线与细菌生长曲线有明显拟合;在有重金属离子存在的污泥体系中,脱氢酶活性可用来作为评价重金属离子的毒性指标;从而脱氢酶活性可取代细菌计数表征污泥活性。

Dehydrogenase activity (DHA) was measured with TTC method using sodium sulfide as reductant and toluene as extractant to investigate the relation of DHA and the logarithm of bacterial number (lgABN) and establish a mathematical model of the characteristic bacterial number of the DHA. The results showed that when the lgABN was between the range of 7.5～12, the sludge activity and lgABN characterized by dehydrogenase assumed obviously linear relation, and the unique linear relation was analyzed theoretically. When the lgABN reached to the logarithmic phase, stable phase, and decline phase at the range of 7.5～12, the DHA converted into the curve drew with the corresponding bacterial number was clearly simulated to the growth curve of the bacteria. When a heavy metal ion existed in the sludge the DHA can be used as the toxicity index of the heavy metal ion, therefore, the DHA can replace the sludge activity characterized by bacterial number.