特应性皮炎和湿疹患者血清白介素4和干扰素γ水平的检测

Detection and Analysis of Serum Levels of Interleukin-4 and Interfero n-gamma in Patients with Atopic Dermatitis and Eczema Before and After Treatme nt

目的探讨血清白介素4(IL-4)和干扰素γ(IFN-γ)水平变化在特应性皮炎、湿疹发病机制中的作用,以及糖皮质激素联合抗生素(治疗组)和单独糖皮质激素(对照组)外用治疗前后两种细胞因子的变化。方法采用双抗体夹心ELISA法检测治疗前后患者血清IL-4和IFN-γ水平结果特应性皮炎、湿疹患者IL-4、IFN-γ水平明显高于正常人对照(P<0.05)。治疗28d后,治疗组IL-4水平明显下降(P<0.05),而IFN-γ无明显变化;对照组两种细胞因子水平变化无统计学意义(P>0.05)。结论特应性皮炎、湿疹患者存在循环IL-4和IFN-γ水平明显异常。糖皮质激素联合抗生素外用治疗特应性皮炎、湿疹可干扰IL-4水平取得较好疗效,较单独外用糖皮质激素疗效显著。

Objectives To study the role of interleukin-4 (IL-4) and interferon-gamma (IFN-γ) in the pathogenesis of atopic dermatitis and eczema,and the changes of serum levels of IL-4 and INF-γ with topical treatment of co rticosteroid plus antibiotic cream or corticosteroid cream alone.Methods Seru m levels of IL-4 and IFN-γ were detected by enzyme-linked immunosorbent assa y(ELISA).Results Serum levels of IL-4 and INF-γ were significantly higher in the patients with atopic dermatitis and eczema than those in the normal control s,respectively(both P < 0.05).In the study group after a 28-day treatment with corticosteroid plus antibiotic cream,serum levels were significantly decreased for IL-4 (P < 0.05)and were no changes for INF-γ.Neither of the two cytokines was changed after the treatment in the controls.Conclusions Both atopic derm atitis and eczema are characterised by abnormality in serum levels of circulatin g IL-4 and INF-γ.Treatment with corticosteroid plus antibiotic cream seems mor e effective,which might influence serum IL-4 level,than that with corticostero id cream alone in atopic dermatitis and eczema.