精浆弹性硬蛋白酶浓度与精浆生化检测结果相关性分析

Analysis of the Correlation between the Concentration of Seminal Plasma PMN-Elastase and the Result of Seminal Plasma Biochemistry Test

目的精浆弹性硬蛋白酶(PMN-Elastase)作为男性生殖系感染的检测指标,男性生殖系受感染后,使精液发生质的变化,通过PMN-Elastase及精浆生化检测结果,判断男性生殖系感染对生育力的影响。方法精浆PMN-Elastase检测用酶联免疫吸附试验(ELISA),精浆生化检测包括:果糖、中性α-葡糖苷酶、酸性磷酸酶、锌。将PMN-Elastase检测结果分为正常组(未受感染)、隐性感染组、显性感染组。并观察与之相应的精浆生化检测结果变化,再进行对比分析。结果279例不育男性,生殖系感染患者97例(PMN-Elastase>200ng/ml),占34.77%。精浆PMN-Elastase浓度随生殖系感染严重程度而升高,果糖、中性α-葡糖苷酶、酸性磷酸酶、锌检测结果则随之降低,呈负相关。结论男性生殖系受到感染时,PMN-Elastase浓度随感染严重程度而增高,精子生成的内环境受到影响,使精液质量降低。从而证明男性生殖系感染可导致不育。

Objective According to seminal plasma PMN-Elastase and the result of seminal plasma biochemistry test,determined affection on fertility when male had reproductive system infection.Methods Seminal plasma PMN-Elastase test used ELISA method.Seminal plasma biochemistry test had four methods,including fructose,neutral alpha-glucosidase,acid phosphatase and zinc.Classified three groups by the result of seminal plasma PMN-Elastase:normal group(uninfection),recessive infection group and dominant infection group.Observed the relevant result of seminal plasma biochemistry test and did comparative analysis.Results Among 279 cases infertility male,97 cases had reproductive system infection (PMN-Elastase>200 ng/ml),taking up 34.77%.When reproductive system infection were serious,the concentration of seminal plasma PMN-Elastase increased while the result of fructose,neutral alpha-glucosidase,acid phosphatase and zinc decreased.Conclusion When male have reproductive system infection,the concentration of PMN-Elastase increase following order of severity of infetion.Consequently can testify reproductive system infection lead to male infertility.