特应性皮炎患者血清白介素-18及-12水平检测及临床意义

The level of IL-12 and IL-18 in the serum of patients with atopic dermatitis

目的:探讨特应性皮炎(AD)患者的血清白介素(IL)-18及IL-12的水平与AD发病的关系及其临床意义。方法:按常规方法作外周血嗜酸性粒细胞(PBEC)绝对计数,采用酶联免疫吸附法(ELISA)测定AD患者和健康对照血清IL-18和IL-12的水平。结果:①AD组与对照组相比,血清IL-18水平和PBEC计数均显著升高(P<0.01),血清IL-12水平显著降低(P<0.01);②AD患者血清IL-18水平及PBEC计数与病情严重程度呈显著正相关(P<0.01),血清IL-12水平与病情严重程度呈显著负相关(P<0.01);③AD患者血清IL-18水平与PBEC计数呈显著正相关(P<0.05),血清IL-12水平与PBEC计数无显著相关性(P>0.05)。结论:AD患者存在免疫紊乱,其血清IL-18水平升高、IL-12水平降低;IL-18的水平升高和PBEC计数增加可能是AD患者病情恶化的指征之一。

Objective: To discuss the role of interleukin-12 (IL-12) and IL-18 in the pathogenesis of atopic dermatitis. Methods: The counting of peripheral blood eosinophil cells (PBEC) was performed by the routine method. Sandwich ELISA was used to measure the level of IL-18 and IL-12 in the serum of the patients and health controls. Results: ①Compared with the control, the IL-12 level significantly decreased (P < 0.01) and the IL-18 level and the amount of PBEC remarkably increased in the patients with atopic dermatitis (P < 0.01). ②the extent of the patient's illness was positively correlated with the level of IL-18 and the amount of PBEC (P < 0.01), and had a negative correlation with the level of IL-12 (P < 0.01). ③ there was a positive correlation between IL-18 and the amount of PBEC (P < 0.05) in the patients, but no correlation was found between IL-12 and the amount of PBEC (P > 0.05). Conclusions: There is an immunity unbalance in the patients with atopic dermatitis. It appears that the increase of IL-18 level and the amount of PBEC and the decrease of IL-12 may indicate the aggravation of atopic dermatitis.