组织激肽释放酶对大鼠阴茎海绵体平滑肌cAMP和cGMP影响的研究

The effects of tissue kallikrein on the concentration of cAMP and cGMP in rat corpus cavernosum

目的探讨组织激肽释放酶对大鼠阴茎海绵体平滑肌cAMP和cGMP的影响,以初步阐明其舒张阴茎海绵体平滑肌的机制。方法采用放射免疫法,测定组织激肽释放酶对培养的大鼠阴茎海绵体平滑肌(去内皮组)和阴茎海绵体平滑肌组织(未去内皮组)cAMP和cGMP的影响,并以硝普钠为阳性对照,以赋形剂为阴性对照。结果100mu组织激肽释放酶使阴茎海绵体平滑肌组织内cAMP和cGMP浓度分别增高2.13倍和2.54倍,而对培养的阴茎海绵体平滑肌cAMP和cGMP没有明显作用。结论组织激肽释放酶能内皮依赖性地提高阴茎海绵体平滑肌细胞内cAMP和cGMP的水平,发挥舒张阴茎海绵体平滑肌的效应。

Objective To investigate the mechanisms of tissue kallikrein in relaxation of rat corpus cavernosum smooth muscle. Methods After tissue kallikrein was incubated with cultured rat corpus cavernosum smooth muscle cells(loss of endothelium) and fresh rat corpus cavernosum tissue(containing endothelium) at 37℃ for 30 min separately, the concentration of cyclic adenosine monophosphate(cAMP) and cyclic guanosine monophosphate(cGMP) were detected by radioimmunoassay. And set sodium nitroprusside as positive control, the equal excipient of kallikrein as negative control. Results 100 mu tissue kallikrein elevated the concentration of cAMP and cGMP in fresh rat corpus cavernosum tissue 2.13 times and 2.54 times respectively, but could not elevate the concentration of cAMP and cGMP in cultured corpus cavernosum smooth muscle cells. Conclusions One of the mechanisms of tissue kallikrein acted on corpus cavernosum was that it could elevate the concentration of cAMP and cGMP simultaneously in an endothelium dependent manner.