摘要
Objective: To obtain sense/antisense eukaryotic expression vectors for human cathepsin L gene,and study the biological effects on human osteosarcoma cell line MG-63 after transfection. Methods:Cathepsin L gene sense/antisense eukaryotic expression vectors were constructed with recombinant technology and transfected into the human osteosarcoma cell line MG-63. The expression of cathepsin L gene mRNA was examined with RT-PCR and the expression of cathepsin L was examined with Western blot. Results:The sense/antisense recombinant eukaryotic expression vectors for cathepsin L were successfully constructed and transfected into MG-63 cell. Conclusion:Antisense cathepsin L gene can significantly inhibit the expression of cathepsin L mRNA and protein.
Objective: To obtain sense/antisense eukaryotic expression vectors for human cathepsin L gene,and study the biological effects on human osteosarcoma cell line MG-63 after transfection. Methods:Cathepsin L gene sense/antisense eukaryotic expression vectors were constructed with recombinant technology and transfected into the human osteosarcoma cell line MG-63. The expression of cathepsin L gene mRNA was examined with RT-PCR and the expression of cathepsin L was examined with Western blot. Results:The sense/antisense recombinant eukaryotic expression vectors for cathepsin L were successfully constructed and transfected into MG-63 cell. Conclusion:Antisense cathepsin L gene can significantly inhibit the expression of cathepsin L mRNA and protein.
