用松枝解剖法快速检测松材线虫病原

Quick detection of Bursaphelenchus xylophilus by the method of dissecting pine twigs

松材线虫Bursaphelenchusxylophilus早期诊断是防治松材线虫病的重要环节。为了在早期快速测定寄主(松树)体内的病原线虫,开发了便携式显微镜和松枝解剖技术。用修枝剪采集松枝,用解剖刀进行松枝切片,再用便携式显微镜镜检,结果显示松枝内松材线虫检出率为70%,其中濒死松树内检出率为100%,局部枯枝内检出率为60%,健康枝内检出率为0。野外松林内鉴定一个样本平均用时2.5min。使携式显微镜加枝条解剖法能对松材线虫病实施早期快速诊断。

Early diagnosis of pine wood nematode(PWN) is a key measure to prevent and control pine wood nematode. To detect PWN in the pine trees early and quickly, the technology of twig dissection with the aid of portable microscope was invented, which could diagnose pine wood nematode quickly. The method was as follows: cutting pine twigs with pruning shears, slicing the twigs up with scalpel, then examining them with the portable microscope. The results showed that the detection rate of nematode was 70% in the infected twigs, 100% in the dying twigs, 60% in partial dead twigs and naught in healthy twigs. The average time for detecting a sample in pine forests was 2.5 minutes. Therefore, the technology of twig dissection with the aid of portable microscope could diagnose pine wood nematode quickly. [Ch, 3 tab. 25 ref.]