高胆固醇兔胆管括约肌细胞钙代谢与钙振荡变化关系

Relationship between calcium metabolism and calcium oscillation of Oddi's sphincter in hypercholesterolemic rabbits

目的:在急性分离的胆管括约肌(sphincterofOddi,SO)细胞上,观察高胆固醇血症对SO细胞内钙振荡的影响变化及其机制.方法:新西兰雌兔24只随机分成两组:对照组和高胆固醇血症模型组各12只,以fluo-4/AM荧光指示剂负载急性分离的SO细胞,应用激光共聚焦扫描显微镜检测细胞钙振荡的变化.结果:高胆固醇血症组兔的SO细胞钙荧光强度振荡的幅度为6.11±3.1,正常组兔为3.61±0.94.当加入硝苯地平及EDTA后试验组钙荧光强度振荡幅度减小到2.24±0.72,下降了64%.当加入thapsigargin后,模型组钙荧光强度振荡幅度减小到3.8±1.9,下降了38%.结论:高胆固醇兔L-型电压依赖钙通道异常开放通过CICR途径所致SO细胞[Ca2+]1代谢紊乱是钙振荡物理学特征改变的分子生物学基础.

AIM: To investigate the effect of hypercholesterolemia on calcium oscillation of acutely separated rabbit Oddi's sphincter muscle cells and to explore its mechanisms. METHODS: Twenty-four New Zealand female rabbits were divided randomly into control group and HC group (12 rabbits each). Sphincter of Oddi's muscle cells were acutely isolated and cultured. The cells were loaded with Fluo-3/ AM, and the changes in calcium oscillation were measured by laser scanning confocal microscopy. RESULTS: Compared with the control group, the amplitude of the Ca2+ oscillation fluorescence intensity was significantly increased in the experimental group (6.11±3.1 vs 3.61±0.94, P<0.05). When treated with nifedipine and EDTA, Ca2+ oscillation amplitude in hypercholesterolemic group was decreased by 64% to 2.24±0.72.When treated with thapsigargin, it was decreased by 38% to 3.8±1.9. CONCLUSION: Calcium metabolic disturbance in Oddi's sphincter muscle cells of hypercholesterolemic rabbits may be caused by the malfunctioned CICR pathway of L-type voltage-dependent calcium channels.