三分群血液分析仪检测中间细胞群增高的复片规则探讨

To discuss the rule of microscopic examination for increased mononuclear in blood with three-classification blod cell analyzer.

目的 探索三分群血液计数仪中间细胞群(mononuclear,MO)增高的复片规则,保证血常规检验质量。方法 收集302例临床血常规标本行全自动血液分析仪检测,同时进行外周血涂片染色镜检并进行对比分析。结果 MO<8%的仪器分类结果与手工镜检分类结果差异无显著性,有良好的相关性。MO>8%的仪器分类结果与手工镜检分类结果差异有显著性,其中有8. 6%的标本手工镜检分类出现异常情况。结论 除了现有的复片规定外,当血液分析仪检测白细胞分类MO为8%以上时,均应作瑞特染色镜检,以防止异型淋巴细胞、嗜酸性粒细胞、白血病细胞等病理性细胞漏检。

Objective To study the rule of microscopic examination for increased mononuclear(MO) in blood with the three-classification blood cell analyzer and assure the quality of blood routine examination. Methods The result of MO in 302 patients were determined by the full-automatic blood cell analyzer and microscopic examination of the blood smear. Results When MO result was <8% in the blood analyzer measurement,there was no significant difference in comparison with microscopic examination of the blood smear,the correlativity was good.When MO was >8%, result was different with microscopic examination.The result of MO>8.6% showed abnormal cell in microscopic examination. Conclusions It should make the Wrights dye microscopic examination,when the level of MO is over 8% with the blood cell analyzer,in order to prevent leak of abnormal cell such as abnormal lymphocyte,eosinophil,leukemia cell etc.