中枢神经系统恶性肿瘤DNA倍性与预后相关性研究

Correlation between DNA ploidy and prognosis of malignant tumors in central nervous system

目的研究中枢神经系统恶性肿瘤DNA倍性和预后的相关性。方法44例肿瘤,包括神经上皮肿瘤、脑膜肿瘤和转移性肿瘤,以肿瘤组织为处理组,瘤周脑组织为对照组。肿瘤和瘤周组织均以胰酶消化法制备成单细胞悬液,碘化丙碇(PI)染色后,流式细胞仪检测细胞DNA倍性。结果正常二倍体肿瘤27例,占61.36%;非正常二倍体肿瘤中异常二倍体14例,占31.82%;四倍体3例,占6.82%。F检验显示,正常二倍体组临床无瘤生存期(6.19±3.37个月)显著长于非正常二倍体组(4.35±4.03个月,P=0.0076)。结论肿瘤细胞DNA倍性和预后存在相关性,可成为多种中枢神经系统恶性肿瘤预后预测指标之一。

Objective To investigate the correlation between DNA ploidy and prognosis of malignant tumors in central nervous system(CNS). Methods Fourty-four cases, including tumors of neuroepithelium, meninges, metastasis, and germioma, were investigated, which were divided into research group and the control (tissue around the tumor) group. All tissues were changed into monocellular suspension by pancreatin digestion. Then DNA ploidy was detected by flow cytometer after propidium iodide (PI) staining.Results Of total 44 cases, 61.36 % were diploidy tumors (27 cases), 31.82% were heterodiploidy (14 cases), and 6.82% were tetraploidy (3 cases ). F-test showed that the average survival time of diploidy cases free of neoplasm (6.19±3.37 months) was much longer than that of heterodiploidy and tetraploidy cases (4.35±4.03 months, P =0.0076). Conclusion There exists a positive correlation between DNA ploidy and prognosis, which means that DNA ploidy can be one of predicting indexes for malignant tumors in central nervous system.