摘要
目的观察雌激素对去卵巢大鼠中脑黑质多巴胺能神经元的作用,探讨雌激素防治帕金森病的可能性。方法成年雌性大鼠行卵巢切除术、随机分为雌激素组(Ⅰ组)和对照组(Ⅱ组),应用立体定向技术注射六羟基多巴胺(6OHDA)于大鼠中脑黑质,采用免疫组织化学的方法对黑质多巴胺能神经元酪氨酸羟化酶(TH)进行标记并记数阳性细胞。同时,对大鼠进行行为学观测。结果在没有给予雌激素的去卵巢大鼠组,阿朴吗啡(APO)诱导的旋转次数与对照组相比有显著增加(P<0.05)、大鼠中脑黑质TH阳性神经元数目与对照组比较也有显著减少(P<0.01)。结论雌激素对雌性大鼠中脑黑质多巴胺能神经元具有保护作用。
Objective To investigate the effects of estrogen on the substantia nigral neuron in female rats which were ovariectomized and to explore the possibility of estrogen to prevent and cure Parkinson disease. Methods All 30 rates were randomly divided into estrogen group (group Ⅰ) and control group (group Ⅱ). Thirty days after 6-hydroxydopamine(6-OHDA) injected stereotaxically into the right side of the mesencephic ventral tegmental area and substantia nigra pars compacta of ovariectomized rats , immunocytochemical techniques was applied to mark the tyrosine hydroxylase (TH) positive cells of the substantia nigra in rats and to count the number of the TH positive cells. Meanwhile the behaviors of rats were observed 30 days after 6-hydroxydopamine (6-OHDA) injected.Results There were significant differences in the number of the TH positive cells and the behaviors of rats in the group without estrogen, comparing with those in control group ( P <0.05).Conclusion Estrogen plays an important role in protecting the substantia nigral dopaminergic neuron of female rats.
出处
《中华神经外科疾病研究杂志》
CAS
2005年第3期233-236,共4页
Chinese Journal of Neurosurgical Disease Research
基金
陕西省科学技术研究发展计划项目(2003K10G91)
