大鼠在空间学习记忆时脑海马区内星形胶质细胞纤维酸性蛋白的表达

Expression of macroglial fibriliary acidic protein in the hippocampus of rats during spatial learning and memory

目的:利用水迷宫试验观察大鼠内海马内星形胶质细胞胶质纤维酸性蛋白的表达在空间辨别性学习记忆时的变化。方法:实验于2003-12/2004-07在暨南大学医学院人体解剖学教研室实验室完成。雄性SD大鼠30只,随机分为对照组、模型5d组和模型15d组,每组10只。采用Morris水迷宫训练,对模型5d组和模型15d组大鼠以定位航行试验建立空间辨别性学习记忆模型,对照组大鼠在无平台的迷宫内自由游泳,2次/d,2min/次,持续5d。各组大鼠停止训练后12h,取脑,冠状连续切片。免疫组织化学S-P法染色以胶质纤维酸性蛋白标记星形胶质细胞。Tiger2000图象分析系统对胶质纤维酸性蛋白的表达进行定量分析。结果:建立了空间辨别性学习记忆模型后,大鼠海马内胶质纤维酸性蛋白的表达模型5d组(CA1区:330.83±36.85;CA3区:333.31±30.37;齿状回区:326.20±36.75)和模型15d组(CA1区:398.33±38.47;CA3区:404.99±48.91;齿状回区:423.55±40.50)高于对照组(CA1区:297.69±27.22;CA3区:296.98±31.13;齿状回区:163.50±33.98),差异有显著性意义(t=2.87～5.37,P均<0.01)。结论:星形胶质细胞参与空间辨别性学习记忆过程并与记忆的进一步巩固有关。

AIM:To observe the changes of macroglial fibriliary acidic protein expression in hippocampus of rats during the learning and memory of spatial discrimination by means of water maze test. METHODS:The experiment was carried out in the laboratory of Staff Room of Huma n Anatomy,Medical College of Jinan University from December 2003 to July 2004.Th irty male SD rats were divided randomly into control group (n=10),5 day model g roup (n=10) and 15 day model group (n=10). Morris water maze training was used, rats in the 5 day and 15 day model groups were made into models of learning an d memory spatial discrimination with the test of localized flying,and rats in th e control group were trained with swimming freely in the maze without platform, twice a day,2 minutes each time for 5 continuous days.The brains of rats in each group were taken 12 hours after they stopped training,coronary slices were prep ared.The tissue was immunohistochemically stained,and astrocytes were labeled wi th glial fibriliary acidic protein.The expression of glial fibriliary acidic pro tein was analyzed quantitatively with the Tiger 2 000 imaging analysis system. RESULTS:After the models of learning and memory spatial discrimination were es tablished,the expressions of glial fibriliary acidic protein in hippocampal regi ons of CA1,CA3 and dentate gyrum were significantly higher in the 5 day model g roup (330.83±36.85,333.31±30.37,326.20±36.75) and 15 day model group (398.33 ±38.47,404.99±48.91,423.55±40.50) than in the control group (297.69±27.22,29 6.98±31.13,163.50±33.98)(t=2.87 to 5.37,P< 0.01). CONCLUSION:Astrocyte plays a crucial role in the learning and memory spatial d iscrimination,and has certain association with the further consolidation of memo ry.