不同类型阿片受体与细胞内信号转导元件-效应器的偶联关系

Association between the opiate receptor of different type and the intracellular signal transduction component-effective apparatus coupling

目的:从目前国内外阿片类药物耐受和依赖及G蛋白与阿片受体偶联的研究出发,探讨G蛋白与阿片受体的偶联关系及在信号传导中的作用。资料来源:应用计算机检索Medline1985-1/2003-12与阿片类药物耐受和依赖及G蛋白偶联相关的文献,检索词“opioiddependenceandtol-erance,G-protein”,并限定文献语言种类为英文。同时计算机检索万方数据库1995-01/2003-12与阿片类药物耐受和依赖的相关文献,检索词“阿片类药物,耐受和依赖,G蛋白”,并限定文献语言种类为中文。资料选择:从资料中选取包括实验组和对照组的文献。纳入标准:①随机对照试验。②对照组为未对阿片类药物形成耐受和依赖。③实验组为均对阿片类药物形成耐受和依赖。排除标准:综述类文献,没有对照组的文献及重复研究的文献。资料提炼:共收集到32篇关于阿片类耐受和依赖的随机对照试验,24篇符合纳入标准。排除的8篇中有6篇为综述,2篇为重复试验。资料综合:阿片类药物作用于阿片类受体后,细胞外信号主要经受体传入胞内是由G蛋白来调节的,胞内有20种以上的G蛋白,不同类型阿片受体与不同种类的G蛋白相互作用。δ和μ受体相偶联的主要是Gi,GO和GZ蛋白(Gi1,Gi2,Gi3,GO1和GO2及),κ受体不仅能与上述G蛋白偶联,而且还能与G16蛋白偶联,但三种受体优先激活的G蛋?

OBJECTIVE:To investigate the coupling relationship between G protein and opia te receptor and its role in signal transduction from the studies of opioid depen dence and tolerance and the coupling with opiate receptor at home and abroad. DATA SOURCES:An online search of Medline was undertaken to identify English li teratures about opioid dependence and tolerance and the coupling with G protein published between January 1985 and December 2003 by using the of 'opi oid dependence and tolerance,G protein'.Meanwhile,Chinese articles related to o pioid dependence and tolerance were searched in Wanfang database published from January 1995 to December 2003 with computer,the keywords were 'opioid dependenc e and tolerance, G protein'. STUDY SELECTION:Literatures including study group and control group were selec ted from the data. Inclusion criteria:①Randomized control trial;②The control g roup was those had no tolerance and dependence on opioid drugs;③The study group was those had tolerance and dependence on opioid drugs.Exclusion criteria:revie ws;literatures without control group;repetitive studies. DATA EXTRACTION:Thirty two randomized control trials about opioid dependence and tolerance were collected, and 24 were in accordance with the inclusion crite ria.Among the 8 excluded papers,6 were review and 2 were repetitive trials. DATA SYNTHESIS:After opioid drugs acted on opiate receptor,the transduction of extracellular signals by receptor into cells was mainly adjusted by G protein, there were more than 20 kinds of G protein in the cells, different opiate recep tors had interactions with different kinds of G protein.δand μreceptors mainl y coupled with Gi, GO and GZ proteins (Gi1,Gi2,Gi3,GO1 and GO2),κreceptor could coupled with not only the above mentioned G proteins,but also G16 ptotein,but the G protein superiorly activated by the 3 receptors were not the same;The en dogenous ligand of orphan receptor was orphanin FQ,its coupling with G protein was not clear, but orphan receptor could directly couple with intracellular effe ctive apparatus by means of non G dependence. CONCLUSION:At present,4 kinds of opiate receptors are known,all the opiate rec eptors can adjust their intracellular effects by G protein.But the coupling bet ween different opiate receptor and G protein are not equal.