健身气功·八段锦对不同性别中老年人一氧化氮、丙二醛和超氧化物歧化酶代谢的影响

Influences of body building Qigong and eight-sectioned brocade on the metabolisms of nitric oxide,malondialdehyde and superoxide dismutase in middle-aged and elderly people of different gender

目的:探讨中小强度有氧训练的健身气功·八段锦对不同性别中老年人自由基代谢的相关指标一氧化氮,丙二醛和超氧化物歧化酶的影响。方法:以2001-07/09黑龙江省哈尔滨市自愿参加健身气功·八段锦锻炼及测试的44人中老年为观察对象。均无严重性疾病,身体健康,不经常参加体育锻炼。按性别与年龄分成4组。①中年组(n=22):男10人,年龄50～60岁;女12人,年龄45～55岁。②老年组(n=22):男11人,年龄61～70岁;女11人,年龄56～65岁。各组每日清晨在辅导员带领下集体练习健身气功·八段锦,50min/d,7次/周。训练10周,锻炼前后取血测试。丙二醛和超氧化物歧化酶水平采用南京建成生物工程研究所提供的试剂盒。血清一氧化氮测试采用解放军总医院放免研究所提供的硝酸盐药盒。检测一氧化氮、丙二醛及超氧化物歧化酶可以反应机体细胞受自由基攻击的严重程度,检测丙二醛用来反映细胞膜脂质过氧化的程度。结果:按意向处理分析,44人均完成10周锻炼,全部进入结果分析。①血清一氧化氮水平:总样本训练后高于训练前[(537.53±200.7),(464.34±153.32)ng/L,P<0.01],老年男子组训练后高于训练前[(558.374±243.91),(471.13±168.12)ng/L,P<0.05],中年男女组及老年女子组训练前后差异不显著。②血清丙二醛水平:总体训练后显著低于训?

AIM:To investigate effect of middle and small intensity aerobics of body build ing Qigong and eight sectioned brocade on the related indexes of free radical m etabolism,including nitric oxide, malondialdehyde and superoxide dismutase,in mi ddle aged and elderly people of different gender. METHODS:Forty four middle aged and elderly people in Harbin city of Heilongj iang Province,who had no severe disease,healthy,and did not often take part in p hysical exercise,voluntarily participated in the training of body building Qigon g eight sectioned brocade and test between July and September 2001.All the subj ects were divided into 4 groups according to their age and gender:①middle aged group(n=22): including 10 males at 50 to 60 years of age and 12 females at 45 t o 55 years of age;②elderly group(n=22): including 11 males at 61 to 70 years of age and 11 females at 56 to 65 years of age.Collective training of body buildin g Qigong and eight sectioned brocade was carried out guided by the instructors every morning in each group,50 minutes per day,7 time every week for 10 weeks.Bl ood samples were drawn and tested before and after experiment.The levels of malo ndialdehyde and superoxide dismutase was detected with the kit provided by Nanji ng Jiancheng Bioengineering Institute,and serum nitric oxide was determined with the nitrite drug kit provided by the Institute of Radioimmunity,General Hospita l of Chinese PLA.The detection of nitric oxide,malondialdehyde and superoxide di smutase could reflect the severity of damage of organic cells caused by the atta ck of free radicals, and the detection of malondialdehyde could be used as an in dex to reflect the degree of cell membrane lipid peroxidation. RESULTS:According to intention to treat analysis,all the 44 subjects complet ed the 10 week exercise training and were involved in the analysis of results. ①The serum level of nitric oxide after training was higher than that before tra ining for the total samples[(537.53±200.7),(464.34±153.32) ng/L,P< 0.01] and t he male elderly group[(558.374±243.91),(471.13±168.12) ng/L,P< 0.05], and ther e was no significant difference before and after training in the male and female middle aged group and the female elderly group.②The serum level of malondiald ehyde after training was lower than that before training for the total samples[( 3.91±0.57), (4.17±0.57) μmol/L, P< 0.05] and the female elderly group[(3.59± 0.45), (4.15±0.68) μmol/L,P< 0.05],and there was no significant difference be fore and after training in the male and female middle aged group and the male e lderly group.③The serum level of superoxide dismutase after training was higher than that before training for the total samples[(1.70±0.064),(1.55±0.25) nkat /L,P< 0.01] and the male elderly group[(1.75±0.17),(1.49±0.22) nkat/L,P< 0.01] ,and there was no significant difference before and after training in the male a nd female middle aged group and the female elderly group. CONCLUSION:The body building Qigong eight sectioned brocade can increase the serum level of nitric oxide,improve the serum activity of superoxide dismutase,d ecrease the serum level of malondialdehyde,and ameliorate the degree of lipid pe roxidation in middle aged and elderly people,so it is good for delaying aging. The improvements of the level of nitric oxide and the activity of superoxide dis mutase are significant in male elderly people,and the decrease of malondialdehyd e level is remarkable in female elderly people,but the differences of the 3 inde xes are not obvious in the male and female middle aged groups.