运动对葡萄糖转运载体蛋白4基因表达的调控

Regulation of glucose transporter 4 gene expression during exercise

目的:探讨运动对葡萄糖转运载体蛋白4基因表达的调控作用。资源来源:应用Medline检索1990-01/2004-01中国医学、生物化学核心期刊关于葡萄糖转运载体蛋白4与运动之间关系研究方面的相关文献,检索词:葡萄糖转运载体蛋白4(GLUT4)、运动(exercise)、肌肉增强因子(MEF2,myocyteenhancerfactor2)、葡萄糖转运载体蛋白4增强因子(GEF,GLUT4enhancerfactor)。资料选择:选择葡萄糖转运载体蛋白4与运动关系的相关文献56篇。资料提炼:在56篇文献中,内容呈不同程度重复的有14篇,给予删除;对42篇文献进行分类整理分析,其中15篇选用为参考文献。资料综合:运动可以增加葡萄糖转运载体蛋白4mRNA的表达,这一机制正在研究中。一次急性运动后葡萄糖转运载体蛋白4的基因转录会短暂激活;经过几天的重复训练后发现葡萄糖转运载体蛋白4蛋白增加两三倍。对葡萄糖转运载体蛋白4启动子的研究结果显示,有两条DNA序列与代谢调节关系密切并使运动应答中基因转录增加。这些DNA元件可与肌肉增强因子和葡萄糖转运载体蛋白4增强因子结合。运动训练时肌肉收缩可激活转录因子(肌肉增强因子,葡萄糖转运载体蛋白4增强因子)进而增加葡萄糖转运载体蛋白4表达。钙调蛋白激酶和AMP激酶活性的升高可使葡萄糖转运载体蛋白4基因转录提高。

OBJECTIVE:To investigate the role of exercise in regulating glucose transporte r 4 gene expression. DATA SOURCES:An online search of Medline was undertaken to identify articles a bout the association between glucose transporter 4 and exercise published in Chi nese medical or biochemical core journals from January 1999 to January 2004 by u sing the of 'glucose transporter 4,exercise,myocyte enhancer factor 2, glucose transporter 4 enhancer factor'. STUDY SELECTION:Fifty six articles about the association between glucose tran sporter 4 and exercise were selected. DATA EXTRACTION:Among the 56 articles,14 with repetitive contents to different degree were deleted,the rest 42 were classified and analyzed,and 17 of them ser ved as references. DATA SYNTHESIS:Exercise could increase the expression of glucose transporter 4 mRNA,and its mechanism was under investigation.Transcription of the glucose tra nsporter 4 gene was transiently activated after an acute bout of exercise,and bo ut protein could be increased as much as two to three times after a few days of repeated exercise.The investigative outcomes of the glucose transporter 4 promot er showed that two sets of DNA sequences were closely correlated with metabolic regulation,and can increase the transcription of the gene in response to exercis e.These DNA elements could combine with the myocyte enhancer factor 2 and glucos e transporter 4 enhancer factor.During exercise,muscle contraction could activat e transcription factors(myocyte enhancer factor 2 and glucose transporter 4 enha ncer factor),and then increase the expression of glucose transporter 4.The incre ased activities of calmodulin kinase and AMP kinase could increase the transcrip tion of glucose transporter 4 gene. CONCLUSION:The activation of the transcription factors (myocyte enhancer facto r 2 and glucose transporter 4 enhancer factor) related to muscle contraction is associated with the increase of the expression of glucose transporter 4 during e xercise,and the mechanism of how to activate these proteins is still the key poi nt of investigation.of 'glucose transporter 4,exercise,myocyte enhancer factor 2, glucose transporter 4 enhancer factor'. STUDY SELECTION:Fifty six articles about the association between glucose tran sporter 4 and exercise were selected. DATA EXTRACTION:Among the 56 articles,14 with repetitive contents to different degree were deleted,the rest 42 were classified and analyzed,and 17 of them ser ved as references. DATA SYNTHESIS:Exercise could increase the expression of glucose transporter 4 mRNA,and its mechanism was under investigation.Transcription of the glucose tra nsporter 4 gene was transiently activated after an acute bout of exercise,and bo ut protein could be increased as much as two to three times after a few days of repeated exercise.The investigative outcomes of the glucose transporter 4 promot er showed that two sets of DNA sequences were closely correlated with metabolic regulation,and can increase the transcription of the gene in response to exercis e.These DNA elements could combine with the myocyte enhancer factor 2 and glucos e transporter 4 enhancer factor.During exercise,muscle contraction could activat e transcription factors(myocyte enhancer factor 2 and glucose transporter 4 enha ncer factor),and then increase the expression of glucose transporter 4.The incre ased activities of calmodulin kinase and AMP kinase could increase the transcrip tion of glucose transporter 4 gene. CONCLUSION:The activation of the transcription factors (myocyte enhancer facto r 2 and glucose transporter 4 enhancer factor) related to muscle contraction is associated with the increase of the expression of glucose transporter 4 during e xercise,and the mechanism of how to activate these proteins is still the key poi nt of investigation.