中药健力方抗运动性疲劳的实验(英文)

Experiment of Chinese herbal jian li fang on anti-kinetic fatigue

背景:补气健脾,补益元气,强筋健骨,疏通经络是中医对抗运动性疲劳的经典原则。目的:观察复方中药抗运动性疲劳的效应。设计:以动物为观察对象的随机对照实验。单位:浙江中医学院动物实验中心。材料:实验于2001-02/06在浙江中医学院动物实验中心完成。选用ICR小鼠80只,雌雄各半,体质量(22±2)g,SD雄性大鼠40只,体质量(194±7)g。方法:①80只小鼠随机分成两组,40只为中药健力方灌胃(实验组),40只为生理盐水灌胃(对照组)。喂养15d后,分成3组进行抗疲劳实验(n=15,游泳时间),抗缺氧能力(n=15),血红蛋白的测定(n=10)。②睾酮测定:把40只大鼠随机分成4组,生理盐水灌胃对照组、中药灌药组、运动组、运动加灌药组。灌药组的大鼠每天灌药1次,运动组及运动加灌药组每天运动1次,为3%体质量负荷游泳至力竭,水温为28℃。喂养20d后,开胸取心血2mL,离心析出血清置4℃冰箱待用,用Minigamma1275γ计数器,VCS法测定。主要观察指标:①各组小鼠的游泳时间测定。②抗缺氧时间。③血红蛋白指标及大鼠睾酮水平。结果:40只大鼠和80只小鼠均进入结果分析。①小鼠游泳时间观察:实验组较对照组明显延长[(125.2±13.7),(108.9±12.6)min,P<0.01]。②抗缺氧能力:实验组较对照组有所提高[(25.0±1.5),(23.1±1.8)min,P<0.01]。③血红蛋?

BACKGROUND:To tonify qi,benefit spleen and primary qi,strengthen tendons and b ones and promote circulations in meridians and collaterals are the classical pri nciples in Chinese medicine for anti kinetic fatigue. OBJECTIVE:To observe the efficacy of compound Chinese herbal medicine on anti kinetic fatigue. DESIGN:Randomized controlled experiment based on animals. SETTINGS:Animal Experimental Center of Wenzhou Normal University and Zhejiang College of Traditional Chinese Medicine. MATERIALS:The experiment was performed in Animal Experimental Center of Zhejia ng College of Traditional Chinese Medicine from February to June 2001,in which,8 0 ICR mice of either sex,body weighted(22±2) g and 40 SD male rats,body weighte d(194±7) g were employed. METHODS:①Eighty mice were randomized into two groups,of which,40 mice was gas tric infused with Chinese herbal jiang li fang(experimental group) and 40 mice was with physiological saline(the control).After bred for 15 days,they were rand omized into 3 groups for anti fatigue experiment(n=15,swimming time),anti hypo xia ability(n=15) and determination of hemoglobin(n=10).②Determination of testo sterone:Forty rats were randomized into 4 groups,named the control with physiolo gical saline infusion,herbal infusion group,exercise group,exercise herbal infu sion group.In herbal infusion group,the rats were infused once a day.In exercise group and exercise herbal infusion group,the rats exercised once a day,swimmin g till being exhausted at the tolerance of 3%body weight at 28 ℃of water tempe rature.After bred for 20 days,heart blood 2 mL was collected with chest opened a nd the serum was centrifuged and placed in refrigerator at 4 ℃. Minigamma 1 275 γcounter and VCS method were applied for determination. MAIN OUTCOME MEASURES:①Determination of swimming time of mice in every group. ②Time of anti hypoxia.③Levels of hemoglobin and testosterone in rats. RESULTS:Forty rats and 80 mice were all entered the result analysis.①Observat ion of swimming time in mice:The time was remarkably prolonged in experimental g roup compared with the control[(125.2±13.7),(108.9 ±12.6) minutes,P< 0.01].②A nti hypoxia ability:It was much improved in experimental group compared with th e control[(25.0±1.5),(23.1±1.8) minutes,P< 0.05].③Level of hemoglobin:The lev el of hemoglobin in experimental group was higher than that in the control,but t he difference was not significant[(162.1±5.2),(159.3±5.3) g/L].④Level of test osterone:That in exercise group of rats was remarkably lower than exercise herb al infusion group[(4.69±0.92),(6.03±0.77) nmol/L, P< 0.05].The difference in t estosterone level was not significant between the control with infusion of physi ological saline and herbal infusion group[(6.59±0.91),(6.98±0.88) nmol/L,P >0. 05]. CONCLUSION:Under fatigue state,jian li fang can prolong the time of swimming i n mice,improve work time and efficacy,increase hemoglobin content and benefit an ti hypoxia ability,which indicates rather strong anti fatigue action.The combi nation of Chinese herbal jian li fang and exercise training can much significant ly increase serum testosterone level in rats compared with exercise group,which explains that jian li fang can increase testosterone level in rats,prevent testo sterone decrease induced by exhausted exercise and accelerate the recovery of ph ysical function.