HPLC-UV测定人血浆瑞芬太尼浓度

Determination of remifentanil in human plasma by HPLC with UV detection

目的建立高效液相色谱-紫外检测法测定人血浆瑞芬太尼浓度。方法人血浆经甲醇沉淀蛋白后先用氯丁烷萃取,再将药物提取至0.01mol·L-1盐酸中。以HypersilCN(4.6mm×250mm,5μm)为固定相;0.02mol·L-1NaH2PO4水溶液-乙腈(70:30),内含三乙胺0.01%为流动相,流速1.5mL·min-1,紫外检测波长为210nm。结果标准曲线在1.0-100ng·mL-1内线性关系良好(r=0.9984)。3种不同浓度平均方法回收率为(113.18±12.26)%,提取回收率为(87.90±3.21)%,日内RSD和日间RSD分别小于10%,15%。结论本方法准确、灵敏、专一性好,适用于临床瑞芬太尼血药浓度检测和药动学研究。

OBJECTIVE: To establish a sensitive method for the determination of remifentanil in human plasma by HPLC with UV detection. METHODS: Remifentanil was extracted with chlorobutane following the precipitation of proteins with methanol. The compound was back extracted into 0.01 mol&middotL-1 HCl again. The separation was performed on a Hypersil CN(4.6 mm × 250 mm, 5 μm) with a mobile phase of 0.02 mol&middotL-1 NaH 2PO4-acetonitrile (70:30)(including 0.01% triethylamine) at a flow rate of 1.5 mL&middotmin-1. The detection wavelength was at 210 nm. RESULTS: A good linearity was obtained over the range of 1.0-100 ng&middotmL-1 (r = 0.998 4). The method recovery was (113.18 ± 12.26)%. The extraction recovery was (87.90 ± 3.21)%. The inter and intra-day RSD were less than 10% and 15% respectively. CONCLUSION: This method was accurate, sensitive and specific for the determination of remifentanil in human plasma in clinical pharmacokinetic and pharmacodynamic study.