摘要
以牛血清白蛋白(BSA) 为模型物质优化共聚物载体的蛋白质吸附量. 选用甲基丙烯酸缩水甘油酯(GMA) 和丙烯酰胺(AA) 为单体, 以N, N′亚甲基双(丙烯酰胺) (MBAA) 为交联剂, 甲酰胺为致孔剂,正庚烷为连续相, 通过双油相悬浮聚合制得一种大孔珠状共聚物载体, 可作为固定化酶或蛋白质纯化介质的载体. 测定了该共聚物载体的比表面积、孔径、孔容分布和环氧基含量. 从过去的实验中知道, 交联度、GMA含量和甲酰胺用量是影响BSA吸附量的主要因素, 因此设计了一组23 析因实验, 分析结果, 各因子的影响显著程度为交联度>GMA用量>甲酰胺用量, 而交互作用均可视为是由误差所引起的. 在此结果上进行的后续实验使树脂对BSA的吸附量从最初的9 1 mg·g-1湿树脂增加到25 6 mg·g-1湿树脂.
The aim of the study was to optimize polymerization conditions so as to maximize the binding capacity for proteins, using BSA as a model substance. A macro-porous hydrophilic bead copolymer was synthesized by organic two-phase suspension polymerization, with glycidyl methacrylate and acrylamide as monomers, N,N'-methylene-bis (acrylamide) as a crosslinker and formamide as the dispersion phase, behaving as porogenic agent, the continuous phase being n-heptane. The characteristic features of the copolymer were studied, including specific surface area, pore diameter, pore volume and distribution, and the content of oxirane groups as well. It was known from past experience that the cross-linking degree, amount of GMA and amount of formamide were the three main factors affecting the BSA adsorption capacity. For exploring their effects and all possible interactions, a 23 complete factorial design was used in experiment. Its analysis showed their effects in the order: cross-linkage > GMA amount > formamide amount, while the three two-factor interactions and the three-factor interaction were attributable to experimental error or noise. Consequently the estimated response (BSA adsorption capacity) could be expressed with a linear function of three main variables. Follow-up investigations found maximum adsorption capacity of 25.6 mg·g-1 wet resin against the original 9.1 mg·g-1 wet resin.
出处
《化工学报》
EI
CAS
CSCD
北大核心
2005年第5期932-936,共5页
CIESC Journal