摘要
对61例膀胱移行细胞癌患者的69价石蜡包埋肿瘤标本进行了流式细胞术(FCM)检测,结果显示肿瘤DNA倍体与肿瘤分级、分期和患者预后关系密切。DNA异倍体肿瘤出现率在病理1级、2级和3级分别为20.0％、64. 9％和72.7％,在1期、2期和3—4期分别为50.0％、68.8％和80.0％。二倍体和近二倍体肿瘤患者5年和10年无瘤生存率为81.5％和61.1％,而异倍体肿瘤患者分别为30.0％和0％。复发病人中,其原发肿瘤与复发肿瘤的DNA含量相关性差。肿瘤S期细胞比例增高表示肿瘤的恶性度高。FCM检测在肿瘤病理分级和分期的基础上能够提供更多预后信息。
Flow cytometry(FCM)was employed for the evaluation of deoxyribonucleic acid ( DNA ) content of 69 pa raffin-embedded bladder tissues obtained from 61 patients.A positive correlation was demonstrated among DNA ploidy,histological grade,stage and clinical outcome in patients with transitional-cell ca rcinoma . The percentages of abnormal DNA patterns were 20.0 per cent,64.9 per cent,72.7 per cent for grade 1 ,grade 2 ,grade 3; and were 50.0 per cent, 68.8 per cent,80.0 per cent for stage 1, stage 2, stage 3-4 , respectively, patients with tumours exhibiting DNA diploid and near diploid patterns had 5 and 10-year rates free of disease of 81. 5 per cent and 61.1 per cent; in contrast, patients with tumours exhibiting abnormal DNA ploidy patterns had 5 and 10-year rates of 30.0 and 0 per cnt, respectively. In the patients suffering from tumour recurrences, the correlation between DNA content of the primary tumours and DNA content of the recurrent tumours was poor.The higher S fraction might demonstrate greater biological malignancy .The results indicated that the DNA ploidy and proliferative activity of the tumours measured by FCM might provide prognostic information beyond that obtained from tumour grade and stage.
