摘要
本工作从健康孕妇水囊引产４～６个月龄的胎儿取肝，采用ＬａＢｒｅｃｑｕｅ方法提取肝刺激因子，称人肝刺激因子（ｈｕｍａｎＨｅｐａｔｉｃＳｔｉｍｕｌａｔｏｒＳｕｂｓｔａｎｃｅ，ｈＨＳＳ）。将ｈＨＳＳ注入肝部分切除之大白鼠体内，用３Ｈ－胸腺嘧啶核苷掺入肝ＤＮＡ法测定其生物活性。结果表明，我们提取的ｈＨＳＳ刺激肝细胞ＤＮＡ合成较对照组增加６倍。采用皮下注射６０％ＣＣｌ４豆油溶液和饮用低浓度的乙醇制备慢性肝损伤动物模型，以观察ｋＨＳＳ对大鼠实验性慢性肝纤维化的影响。实验结果表明，（１）ｈＨＳＳ可以明显降低ＣＣｌ４－乙醇所致慢性肝损伤时肝组织羟脯氨酸含量的升高，在实验第２８、４２天差异具显著性（Ｐ＜０．０５，０．０１）。（２）病理组织学观察表明，ｈＨＳＳ可以明显降低ＣＣｌ４－乙致慢性肝损伤时肝组织纤维化的程度。在实验的第４２天差异具显著性（Ｐ＜０．０１）。本工作结果提示，肝再生刺激因子可能是一种有希望应用于临床治疗慢性肝病的生物制剂。
Human hepatic stimulator substance(hHSS) was extracted from the liver induced labor fetus from 4-6 months healthy pregnant women according to the method of LaBrecque.The bioactivity of hHSS was detected by determination of the rate of 3H-thymidine.incorporation into the hepa tocyte DNA after injection of hHSS into the abdominal cavity of partial hepatectomized rats.The results showed that the amount of DNA syn thesis stimulated by hHSS was 6 times as much as that of control group.The model of experimental chronic liver injury was established by injec tion of carbon tetrachloride(CCl4)hypodermically and feeding of alcohol.The experimental results showed:(1) hHSS could lessen the elevation of the liver HYP content in the chronic CCl4-alcohol intoxicated rats at the 28th and 42th days during the period of chronic liver injury.(2) Pathohistological finding indicated:hHSS could remarkably reduce the degree of liver fibrosis induced by chronic CCl4-alcohol injury in rats.
关键词
人胎肝刺激因子
肝纤维化
四氯化碳
乙醇
human hepatic stimulator substance(hHSS)
liver fibrosis
Carbon tetrachloride (CCl_4)
alcohol
hydroxyproline(HYP)
