真菌诱导物对丹参转化细胞几种氧化酶活性的影响

The Effects of Fungi Inducers on Several Oxidases in Transformed Salvia miltiorrhiza Cell

用大丽轮枝菌(VerticilliumdahliaeKleb.)激发子V44和酵母提取物y-e分别诱导悬浮培养的丹参(Salviamiltiorrhiza)转化细胞,发现丹参转化细胞过氧化物酶、过氧化氢酶和多酚氧化酶活性均随诱导时间和诱导剂浓度的增加而改变。结果表明:大丽轮枝菌激发子的作用强于酵母提取物,y-e+V44联合诱导作用强于单一激发子刺激。

Transformed Salvia miltiorrhiza cell suspension cultures were induced by Ver- ticillium dahlium V44 and yeast-extract respectively(y-e).The activities of peroxidase, catalase and polyphenolic oxidase of transformed Salvia miltiorrhiza cell change with the increase of the inducing time and inducing dosage.The effect of the elicitor from Ver- ticillium dahlium on the transformed Salvia miltiorrhiza cell is stronger than the yeast extract;y-e+V44 combination has a stronger inducing effect than the single stimulus.