半夏泻心汤及其拆方对老年胃动力作用机理实验研究

Experimental Study of the Mechanism of Banxiaxiexin Decoction and Its Different Ingredient Combinations on Aged Gastric Motility

目的:半夏泻心汤及其拆方对老年胃动力作用的机理。方法:选取老年大鼠40只,随机分为5组,分别以半夏泻心汤辛开、苦降、甘润和全方组相应药液及生理盐水灌胃,检测胃内残留率、血浆胃动素、胃泌素和一氧化氮含量,观察半夏泻心汤不同配伍对上述指标的影响。结果:甘润组和全方组血浆胃动素、胃泌素和一氧化氮含量显著升高,胃内残留率显著降低(P<0.05或P<0.01);苦降组血浆胃泌素和一氧化氮含量较对照组有所增高(P<0.05),但血浆胃动素、胃内残留率与对照组比较没有显著性差异(P>0.05);辛开组血浆胃泌素和一氧化氮含量与对照组比较没有显著性差异(P>0.05),血浆胃动素含量显著降低(P<0.01),胃内残留率显著高于对照组(P<0.05)。结论:半夏泻心汤全方组增加血浆胃动素、胃泌素和一氧化氮的含量,调节老年胃动力的作用最为显著,充分体现了经方的科学性、合理性和配伍严谨的特点。

Objective:To study the mechanism of Banxiaxiexin Decoction and its different ingredient combinations on aged gastric motility.Methods:40 aged rats were divided into XinKai group, KuJiang group, GanRun group, Banxiaxiexin Decoction group and the control group randomly. Each group included 8 aged rats. XinKai decoction, KuJiang decoction, GanRun decoction, Banxiaxiexin Decoction and saline water had been given separately for 3 weeks. Then the contents of the gastric remnant rate, motilin, gastrin and nitric oxide in blood serum in each group were detected.Results:The contents of motilin, gastrin and nitric oxide in blood serum in GanRun group and Banxiaxiexin decoction group significantly increased, and the gastric remnant rate significantly decreased (P<0.05 or P<0.01). The contents of gastrin and nitric oxide in blood serum in KuJiang group were also significantly increased(P<0.05). There was no significant difference in the gastric remnant rate and the contents of motilin between KuJiang group and the control group(P>0.05). There was no significant difference in the contents of gastrin and nitric oxide in blood serum between XinKai group and the control group(P>0.05). The contents of motilin in blood serum significantly decreased in XinKai group(P<0.01). The gastric remnant rate also significantly increased in XinKai group(P<0.05).Conclusion:The contents of motilin, gastrin and nitric oxide in blood serum in GanRun group and Banxiaxiexin Decoction group most significantly increased. The study proved that the classical decoction is scientific, rational and rigorous and GanRun herb is very important to regulate the aged gastric motility.