比色法测定胃乐益冲剂中薯蓣皂苷元的含量

Determination of Diosyenin by Colorimetry in the Weileyi Granules

目的建立胃乐益冲剂中薯蓣皂苷元的含量测定方法.方法采用紫外分光光度法,最大吸收波长406 nm.结果薯蓣皂苷元在0.002 4～0.012 0 mg·mL-1浓度范围内与吸光度呈良好的线性关系,r＝0.999 7(n＝5),平均回收率为98.37%,RSD＝2.25%(n＝5).结论该方法简便快速,灵敏度高,重现性好,适用于该制剂的质量控制.

Objective To establish a colorimetry method for the determination of diosyenin in weileyi granules. Methods The diosyenin was determined by ultraviolet spectrophotometry. The detecting wavelength was 406 nm. Results A good linearity was shown within the range of 0.002 4-0.012 0 mg·mL -1 ( r =0.999 7). The average recovery was 98.37%, RSD =2.25%( n =5). Conclusion This method is rapid and accurate. Its repeatability is good, and it could used to for the quality control of the weileyi granules.