油菜菌核病拮抗细菌的筛选和高效菌株的鉴定

Isolation and identification of biocontrol bacteria from rhizosphere of rapeseed

从油菜根际和叶围分离得到320个细菌分离物,在马铃薯葡萄糖琼脂培养基上的拮抗实验中,18个分离细菌表现对油菜菌核病菌不同程度的拮抗作用,其中Y1菌株对油菜菌核病菌菌丝的生长具有明显的抑制作用。对Y1进行油菜离体叶片、温室盆栽和田间小区接种实验,该菌均表现对油菜菌核病明显的防病效果;在温室盆栽试验和田间小区试验中,防病效果达到92%。经过鉴定,Y1菌株为枯草芽孢杆菌。

Three hundred and twenty bacteria isolates were isolated from rhizospere and phyllophere of rapeseed, 18 of the isolates were found suppressing Scleotinia sclerotiorum mecylium growth on the PDA plate, among them Y1 was found efficiently suppress Scleotinia sclerotiorum. Y1 was also found efficiently suppress Scleotinia sclerotiorum in biocontrol test on rapeseed detached leaves, seedling in the greenhouse and field plot. The efficacy of 92% was obtained in biocontrol test in the greenhouse and field plot. This bacteria isolate was identified as Bacillus subtilis.