慢性乙型肝炎活动期HBV复制与血清β_2-MG变化的关系

The Relations between the Replication of HBV and the Changes of the Beta-2-Microglobulin Levels in Patients with Acute Exacerbation of Chronic Hepatitis B

目的探讨慢性乙型肝炎活动期HBV复制与血清β2MG变化的关系。方法75例慢性乙型肝炎活动期患者和61例正常体检者采用荧光定量PCR法(FQ-PCR)测定血清HBV-DNA的水平,放射免疫法(RIA)测定血清β2MG的水平,并同时行肝功能及病毒性肝炎标志物检测。结果慢性乙型肝炎组血清β2MG水平显著高于正常对照组(P<0.05),HBV-DNA阳性组血清β2MG水平显著高于HBV-DNA阴性组(P<0.01),HBV-DNA阴性组血清β2MG水平与正常对照组比较,无显著性差异(P>0.05)。结论(1)慢性乙型肝炎活动期患者血清β2MG水平升高,是病情活动的指标之一;(2)联合检测血清HBV-DNA和血清β2MG对于观察病情和评估疗效有重要的临床应用价值。

Objective To investigate the change of serum beta-2-microglobulin levels in acute exacerbation of chronic HBV patients with serum HBV-DNA positive or negative.Methods Seventy-five patients with acute exacerbation of chronic hepatitis B were collected in the study, and sixty-one normal adult as control group.Serum HBV DNA levels were quantitatively determined using fluorescent quantitative polymerase chain reaction(FQ-PCR) and serum beta-2-microglobulin levels were measured by radioimmunoassay(RIA).Serological markers of viral hepatitis as well as other parameters relevant to liver function were also determined.Results Serum beta-2-microglobulin levels were significantly higher than that in normal control group (P<0.05).Serum beta-2-microglobulin levels were significantly higher in HBV DNA-positive group vs. HBV DNA-negative group(P<0.01).Serum beta-2-microglobulin levels were no difference between HBV DNA-negative group and normal control group(P>0.05).Conclusion (1)There exists higher serum beta-2-microglobulin levels in the patients with acute exacerbation of chronic hepatitis B;(2)The determination of serum HBV-DNA and serum beta-2-microglobulin levels is clinically useful for observing the state of the illness and evaluating the therapeutic effect of chronic hepatitis B.