牛磺酸对东莨菪碱所致记忆获得性障碍模型小鼠的改善作用

Effect of Taurine on learning-memory ability and M-cholinergic receptor in dementia model mice induced by scopolamine

目的观察牛磺酸对东莨菪碱所致模型小鼠学习记忆及对其脑组织M胆碱受体结合力的影响。方法将昆明小鼠随机分组,设立正常对照组,东莨菪碱模型组及阳性对照药(吡拉西坦)组,牛磺酸小剂量组(0.3g·kg-1),中剂量组(0.75g·kg-1),大剂量(1.875g·kg-1)组,分别灌胃给药,于d5,除正常对照组腹腔注射生理盐水外,其它各组注射东莨菪碱1mg·kg-1,20min后进行Morris水迷宫测试,测试5d后处死,取其脑组织进行M受体结合力测定。结果在行为学实验中,牛磺酸各剂量组游出时间与模型组相比有明显缩短(P<0.05),并可以提高模型小鼠M胆碱受体的结合力。结论牛磺酸对东莨菪碱所致模型小鼠学习记忆障碍及M受体有一定的改善作用。

Aim To observe the effect of taurine on learning-memory ability and its mechanism in model mice induced by scopolamine(Scop). Methods Kunming mice were randomly divided into normal control group, Scop model group, taurine groups (0.3, 0.75, 1.875 g·kg -1) and Piracetam group (positive control group). The drugs were continually intra-gastrically administrated for 5 days. On day 5, after intraperitoneal injection of scopolamine, mice were subjected to Morris water maze test. 5 days later, M-cholinregic receptor binding was assayed by radio ligand binding test.Results Compared with model group, significant decrease of swimming time and distance in Morris water maze test was observed in all 3 taurine groups. Taurine at middle dose increased M-cholinergic receptor binding in the brain of model mice. Conclusion Taurine effectively improves learning-memory ability in model mice induced by scopolamine injection. The mechanism relates with the improvement of M-cholinergic receptors of brain.