大豆磷酸烯醇式丙酮酸磷酸酯酶(PEPP)研究:I.对非生物胁迫的反应

Studies on soybean phosphoenolpyruvate phosphatase(PEPP):I. Response to abiotic stresses

对缺磷、铝毒、盐、干旱、Fe、Mn、Cu、Zn胁迫下大豆根系和叶片磷酸烯醇式丙酮酸磷酸酯酶(PEPP)活性进行了测定,结果表明:(1)长期缺磷和铝毒胁迫可显著提高大豆根系的PEPP活性,并表现出加成效应;(2)长期铝毒、缺磷和铝毒耦合胁迫也显著提高大豆敏感品种BL2叶片的PEPP活性;(3)盐胁迫、干旱以及长期Fe、Mn、Cu、Zn过量都显著提高大豆根系的PEPP活性,Fe、Cu、Zn缺乏则对根系和叶片的PEPP活性影响不大,长期缺Mn显著降低大豆根系及叶片的PEPP活性。

This experiment was conducted with two soybean species of BX10 and BL2,to estimate phosphoenolpyruvate phosphatase(PEPP) activities of soybean leaves and roots under abiotic stresses of P deficiency,Al toxicity,salt excess,drought,microelements(Fe,Mn,Cu,Zn) excess as well as deficiency.The results showed that:(1) 14 d period stresses of both P deficiency and Al toxicity resulted in significant and additive increasing in root PEPP activities;(2) both Al toxicity and its combination with P deficiency stresses significantly increased leaves PEPP activities of BL2,the sensitive variety to P deficiency and Al toxicity;(3)salt excess,drought and 14d period stresses of microelement (Fe,Cu,Mn,Zn) excess other than deficiency significantly increased both roots and leaves PEPP activities,however,14d period stress of Mn deficiency significantly decreased roots and leaves PEPP activities.