腹膜透析患者透出液透明质酸的变化

Changes of hyaluronan concentration in the peritoneal effluent of patients treated vtitfa peritoneal dialysis

目的观察腹膜透析(简称腹透)患者透出液透明质酸(HA)的变化。方法对22例尿毒症腹透患者的透出液采用放射免疫法测定HA水平。结果腹透患者透出液HA较血清HA水平明显增高,分别为(665.79±562.81)μg/L和(336.46±235.32)μg/L(P<0.05),而层黏连蛋白(LN)和Ⅲ型前胶原(PCⅢ)水平无显著差异;腹透患者透析时间大于3年组较小于3年组的透出液HA水平明显减低,分别为(211.16±73.82)μg/L和(410.10±96.35)μg/L(Jp<0.005),而LN和PCⅢ水平无显著差异;腹透患者腹膜炎组透出液HA水平较非腹膜炎组显著增高,分别为(845.12±219.68)μg/L和(303.38±117.27)μt/L(P<0.005);腹膜炎组透出液LN及PCⅢ也明显增高(P<0.01)。结论测定腹透患者透出液HA水平有助于了解腹膜间皮细胞功能及腹腔状念。

Objective To observe hyalurome changes of the peritoneal effluent from patients treated with peritoneal dialysis. Methods Concentration of hyaluronan in the peritoneal effluent and serum of the patients treated with peritoneal dialysis was measured by radio-immunoassay kits. Results Concentration of hyaluronan in the peritoneal eflluent was significantly higher than that of their serum [ (665.79 ± 562.81)μg/L ps (336.46 ± 235.32) ,μg/L, P < 0.05], but the concentrations of Laminin and type Ⅲ precollagen did not increase significantly. As compared with group of more than 3 years, concentration of hyaluronan in the peritoneal effluent of group of less than 3 years was significantly lower [ (211 . 16 ±73.82) /μg/L vs (410. 10± 96.35) μg/L, P < 0.005], but The concentrations of Laminin and type Ⅲ pre-collagen did not reduce significantly. Concentration of hyaluronan in the peritoneal effluent of peritonitis group was significantly higher than that of non-peritonitis group (845. 12 ± 219.68) μg/L vs (303.38±117.27) μg/L, P < 0.005], and the concentrations of Laminin and type Ⅲ precollagen in the peritoneal effluent of non-peritonitis group were significantly increased (P < 0.01). Conclusion These'data indicate that the measurement of hyaluronan in the peritoneal effluent of the patients treated with peritoneal dialysis is helpful for understanding the function of peritoneal mesothelial cells and the state of the peritoneal cavity.