进出口植物产品中甲霜灵残留量酶联免疫检测试剂盒的研究

Research of ELISA kit for screening Metalaxyl residues in importing and exporting plants

目的本研究采用直接竞争ELISA法对进出口植物产品中甲霜灵残留量进行快速筛选。方法将甲霜灵转化成甲霜灵酸,利用适当的方法将其与辣根过氧化物酶(HRP)进行偶联,制备酶标半抗原。采用传统的盐析法提取IgG并建立了甲霜灵直接竞争ELISA快速检测方法。结果抑制试验表明其检测范围在(0.78-50.0)ng/mL之间,可用于进出口植物产品中甲霜灵的定量检测。该方法与气相色谱检测符合率为94%。样品的平均回收率为94%。交叉反应显示该抗体仅与甲霜灵、甲霜灵酸起反应,不与结构相似的毒草胺、甲草胺、异丙甲草胺、新燕灵和己酰甲草胺起交叉反应。结论本试剂盒具有操作简便、快速、灵敏等特点。

Objective To screen Metalaxyl residues in importing and exporting plants by direct competitive ELISA method. Methods Metalaxyl was acidified to its acid, and then the HRP-Metalaxyl conjugate was prepared by coupling the acid to Horseradish Peroxidase using a water-soluble carbodiimide procedure. IgG was extracted by traditional method [Saturated (NH4 )2SO4 ] , and then the ELISA method lor Metalaxyl was set up. Results The detection area ranged from 0.78 ng/mL to 50.0 ng/mL was fit for the demand of quantitative determination of Metalaxyl residue in plant productions. The coincidence rate between the direct competitive method and the gas chromatography was 94% . The average recovery of fortified samples was 94% . Cross-reactivity indicated that the antiserum could only react with Metalaxyl and its acid, but not Metolachlor, Diethatyl ethyl, Alachlor Propachlor, or Benzoylprop-ethyl, whose structures are similar to Metalaxyl. Conclusion This testing kit is simple, rapid, and sensitive.