摘要
目的探讨端粒酶催化亚基基因(hTERTmRNA)在口腔鳞癌患者中的表达情况及其意义。方法采用实时定量PCR法(qRT-PCR)检测25例鳞癌患者口腔鳞癌组织及其切缘组织,10例正常人口腔黏膜中hTERTmRNA的表达情况并进行比较。结果口腔鳞癌组织中hTERTmRNA的表达量明显高于切缘组织和正常口腔黏膜(P<0.05),而切缘组织与正常口腔黏膜中hTERTmRNA的表达量无区别(P>0.05)。结论口腔鳞癌中hTERT高表达,口腔切缘和正常口腔黏膜中低或无表达,提示hTERTmRNA活化是口腔癌变过程中的重要事件,hTERTmRNA可以作为检测口腔鳞癌及其切缘安全范围的一个客观量化指标。
Objective To investigation the expression of human telomerase reverse transcriptase (hTERT) mRNA in oral squamous cell carcinomas and its clinical significance. Methods Tissue specimens were collected from 25 cases of oral squamous cell carcinoma, including cancer tissue and corresponding tissue of surgical margin, 10 specimens of normal mucosa were used as control. hTERT was detected by real time quantitative RT-PCR in all 60 specimens. Results The quantity of hTERTmRNA in oral squamous cell carcinoma was higher than that of margin tissue and normal mucosa (P<0.05),no difference was found between margin tissues and normal mucosa (P>0.05).Conclusion The quantitative evaluation of hTERT mRNA could be an important marker for safety surgical margin and of prognostic significance in human oral cancer.
出处
《浙江医学》
CAS
2005年第6期410-411,414,共3页
Zhejiang Medical Journal
基金
浙江省卫生厅卫生科技基金(2003158B)
